Freshwater ice as habitat: partitioning of phytoplankton and bacteria between ice and water in central European reservoirs.

Environmental microbiology reports

PubMedID: 26224255

McKay RM, Prášil O, Pechar L, Lawrenz E, Rozmarynowycz MJ, Bullerjahn GS. Freshwater ice as habitat: partitioning of phytoplankton and bacteria between ice and water in central European reservoirs. Environ Microbiol Rep. 2015;.
Abundant phytoplankton and bacteria were identified by high-throughput 16S rRNA tag Illumina sequencing of samples from water and ice phases collected during winter at commercial fish ponds and a sand pit lake within the UNESCO Trebon Basin Biosphere Reserve, Czech Republic. Bacterial reads were dominated by Proteobacteria and Bacteroidetes. Despite dominance by members of just two phyla, UniFrac principal coordinates analysis of the bacterial community separated the water community of Klec fish pond, as well as the ice-associated community of Klec-Sand Pit from other samples. Both phytoplankton and cyanobacteria were represented with hundreds of sequence reads per sample, a finding corroborated by microscopy. In particular, ice from Klec-Sand Pit contained high contributions from photoautotrophs accounting for 25% of total reads with reads dominated by single operational taxonomic units (OTUs) of the cyanobacterium Planktothrix sp. and two filamentous diatoms. Dominant OTUs recovered from ice were largely absent (