Novel bimodal iron oxide particles for efficient tracking of human neural stem cells in vivo.

Nanomedicine (London, England)

PubMedID: 26296195

Aswendt M, Henn N, Michalk S, Schneider G, Steiner MS, Bissa U, Dose C, Hoehn M. Novel bimodal iron oxide particles for efficient tracking of human neural stem cells in vivo. Nanomedicine (Lond). 2015;10(16):2499-512.
AIMS
We validated novel bimodal iron oxide particles as substitute of ferumoxides for efficient labeling of human neural stem cells (NSCs). The dextrane-coated FeraTrack Direct (FTD)-Vio particles have additional far-red fluorophores for microscopic cell analysis.

METHODS
MR relaxometry, spectrophotometric iron determination and microscopy are used for characterization in vitro and in vivo.

RESULTS
Efficient uptake is not transfection agent-dependent. FTD-Vio594 labeling had no influence on viability, proliferation, migration and differentiation capacity. It allows MRI-based tracking of engrafted NSCs in mouse brain up to 11 days, complemented by bioluminescence imaging of firefly luciferase expressed by the engrafted cells.

CONCLUSION
Our results highlight the FTD-Vio594 particles as safe and sensitive substitute of ferumoxides for longitudinal tracking of NSCs in preclinical studies.