Isolation and expansion of cytokeratin positive progenitor cells from adult murine pancreatic ducts expressing Pdx-1, Nestin, Sox9, MafA and hepatic nuclear factors.

Minerva endocrinologica

PubMedID: 26337491

Grabmaier U, Rottstegge I, Offers M, Ziegler T, Brenner C, Huber BC, Seissler J. Isolation and expansion of cytokeratin positive progenitor cells from adult murine pancreatic ducts expressing Pdx-1, Nestin, Sox9, MafA and hepatic nuclear factors. Minerva Endocrinol. 2015;.
AIMS
Recent studies suggest that stem cells may represent a putative source for the generation of beta cells. However, the identity and characteristics of stem cells from adult pancreas and conditions for their large scale expansion are still poorly defined.

MATERIALS AND METHODS
DPC were isolated from adult pancreatic ducts of C57Bl/6 mice. Expression profile was investigated by PCR, FACS and immunohistochemistry.

RESULTS
DPC express a panel of stem cell associated markers such as Pdx-1, cytokeratin-19 (CK19), Nestin, Sox9 together with the transcription factor MafA and hepatic nuclear factors HNF1ß, HNF3ß, HNF4? und HNF6. This gene expression profile is suggesting that DPC might be a promising tool for endocrine differentiation. After stimulation with picolinic acid and hypoxia, DPC expressed the endocrine differentiation marker Ngn3. Nevertheless, insulin production was not observed.

CONCLUSION
We here describe a protocol for the isolation end expansion of murine pancreatic ductal progenitor cells (DPC) displaying high self-renewal, spheroid- and colony-forming capacity. Further studies are required to elucidate the conditions for differentiation into mature pancreatic endocrine cell lineages.