Fast 3-D temporal focusing microscopy using an electrically tunable lens.

Optics express

PubMedID: 26406641

Jiang J, Zhang D, Walker S, Gu C, Ke Y, Yung WH, Chen SC. Fast 3-D temporal focusing microscopy using an electrically tunable lens. Opt Express. 2015;23(19):24362-8.
In this paper, we present a 3-D temporal focusing microscope based on an electrically tunable lens (ETL) and a femtosecond regenerative laser amplifier. The focus-tunable lens provides a fast and compact way to perform non-mechanical z-scanning and resolves the blurry image issue compared with GVD-based z-scanning methods. The optical performance of the temporal focusing system, including z-scanning characteristics, the associated the magnification variation, and the lateral and axial resolution, has been studied and characterized using calibrated Rhodamine-6G thin film sample, fluorescent beads, and pollen samples. Lastly, we demonstrate the optical cross-sectioning and z-scanning capability with an in vivo experiment, where Ca2+ imaging of neurons in GaCamp6 labeled zebrafish was performed.