One step electrodeposition of dendritic gold nanostructures on ß-lactoglobulin-functionalized reduced graphene oxide for glucose sensing.

Talanta

PubMedID: 26452896

Du X, Zhang Z, Miao Z, Ma M, Zhang Y, Zhang C, Wang W, Han B, Chen Q. One step electrodeposition of dendritic gold nanostructures on ß-lactoglobulin-functionalized reduced graphene oxide for glucose sensing. Talanta. 2015;144823-9.
Dendritic gold nanostructures (AuNDs) were successfully synthesized by one step electrodeposition on reduced graphene oxide (rGO) functionalized by a globular protein, ß-lactoglobulin (BLG), for the first time. Owing to its sulfhydryl groups, water-soluble BLG-rGO provided a superb platform for the growth of AuNDs. Scanning electron microscopy, Raman spectroscopy and X-ray spectroscopy analysis were used to investigate the as prepared BLG-rGO-AuNDs nanocomposite. Electrocatalytic ability of the nanocomposite was evaluated by cyclic voltammetry and chronoamperometric method. In order to prove the superiority of BLG-rGO-AuNDs, we developed a novel glucose biosensor on the nanocomposite modified glassy carbon electrode (GCE) through a cross-linking method. The biosensor exhibited a remarkable sensitivity of 46. 2µAmM(-1)cm(-2), a wide linear range of 0. 05-6mM glucose, a low detection limit of 22. 9µM (S/N=3), and a rapid response time (within 6s). The prepared biosensor also used to detect glucose in human serum and statistical analysis in the respect of reproducibility was done.