Identification of C(2)M interacting proteins by yeast two-hybrid screening.

Yi chuan = Hereditas / Zhongguo yi chuan xue hui bian ji

PubMedID: 26582530

Shanshan Y, Laixin X. Identification of C(2)M interacting proteins by yeast two-hybrid screening. Yi Chuan. 2015;37(11):1160-6.
The synaptonemal complex (SC) is a huge structure which assembles between the homologous chromosomes during meiotic prophase I. Drosophila germ cell-specific nucleoprotein C(2)M clustering at chromosomes can induce SC formation. To further study the molecular function and mechanism of C(2)M in meiosis, we constructed a bait vector for C(2)M and used the yeast two-hybrid system to identify C(2)M interacting proteins. Forty interacting proteins were obtained, including many DNA and histone binding proteins, ATP synthases and transcription factors. Gene silencing assays in Drosophila showed that two genes, wech and Psf1, may delay the disappearance of SC. These results indicate that Wech and Psf1 may form a complex with C(2)M to participate in the formation or stabilization of the SC complex.