Transient Protein Expression by Agroinfiltration in Lettuce.

Methods in molecular biology (Clifton, N.J.)

PubMedID: 26614281

Chen Q, Dent M, Hurtado J, Stahnke J, McNulty A, Leuzinger K, Lai H. Transient Protein Expression by Agroinfiltration in Lettuce. Methods Mol Biol. 2015;138555-67.
Current systems of recombinant protein production include bacterial, insect, and mammalian cell culture. However, these platforms are expensive to build and operate at commercial scales and/or have limited abilities to produce complex proteins. In recent years, plant-based expression systems have become top candidates for the production of recombinant proteins as they are highly scalable, robust, safe, and can produce complex proteins due to having a eukaryotic endomembrane system. Newly developed "deconstructed" viral vectors delivered via Agrobacterium tumefaciens (agroinfiltration) have enabled robust plant-based production of proteins with a wide range of applications. The leafy Lactuca sativa (lettuce) plant with its strong foundation in agriculture is an excellent host for pharmaceutical protein production. Here, we describe a method for agroinfiltration of lettuce that can rapidly produce high levels of recombinant proteins in a matter of days and has the potential to be scaled up to an agricultural level.