A game of tag: MAPS catches up on RNA interactomes.

RNA biology

PubMedID: 26967018

Carrier MC, Lalaouna D, Massé E. A game of tag: MAPS catches up on RNA interactomes. RNA Biol. 2016;0.
In the last few decades, small regulatory RNA (sRNA) molecules emerged as key regulators in every kingdom of life. Resolving the full targetome of sRNAs has however remained a challenge. To address this, we used an in vivo tagging MS2-affinity purification protocol coupled with RNA sequencing technology, namely MAPS, to assemble full bacterial small RNAs targetomes. The impressive potential of MAPS has been supported by a number of reports. Here, we concisely overview RNA-tagging history that preceded the development of the MAPS assay and expose the range of possible uses of this technology.