A digital microfluidic interface between solid-phase microextraction and liquid chromatography-mass spectrometry.

Journal of chromatography. A

PubMedID: 27048987

Choi K, Boyaci E, Kim J, Seale B, Barrera-Arbelaez L, Pawliszyn J, Wheeler AR. A digital microfluidic interface between solid-phase microextraction and liquid chromatography-mass spectrometry. J Chromatogr A. 2016;14441-7.
We introduce a method to couple solid-phase microextraction (SPME) with HPLC-MS using digital microfluidics (DMF). In the new system, SPME fibers are used to extract analytes from complex sample solutions, after which the analytes are desorbed into solvent droplets in a DMF device. The open geometry of DMF allows straightforward insertion of SPME fibers without requiring a complicated interface, and automated droplet manipulation enables multiplexed processing of the fibers. In contrast to other multiplexed SPME elution interfaces, the low volumes inherent to DMF allow for pre-concentration of analytes prior to analysis. The new SPME-DMF-HPLC-MS method was applied to the quantification of pg/mL-level free steroid hormones in urine. We propose that this new method will be useful for a wide range of applications requiring cleanup and pre-concentration with convenient coupling to high-performance analytical techniques.