Derivation of human embryonic stem cell line Genea023.

Stem cell research

PubMedID: 27346015

Dumevska B, Bosman A, McKernan R, Goel D, Schmidt U, Peura T. Derivation of human embryonic stem cell line Genea023. Stem Cell Res. 2016;16(2):456-9.
The Genea023 human embryonic stem cell line was derived from a donated, fully commercially consented ART blastocyst, through ICM outgrowth on inactivated feeders. The line showed pluripotent cell morphology and genomic analysis verified a 46, XY karyotype and male allele pattern through CGH and STR analysis. Pluripotency of Genea023 was demonstrated with 85% of cells expressed Nanog, 98% Oct4, 55% Tra1-60 and 98% SSEA4, gave a Pluritest Pluripotency score of 42. 76, Novelty of 1. 23, demonstrated Alkaline Phosphatase activity and tri-lineage teratoma formation. The cell line was negative for Mycoplasma and visible contamination.