Derivation of Genea052 human embryonic stem cell line.

Stem cell research

PubMedID: 27345996

Dumevska B, Chami O, McKernan R, Goel D, Schmidt U. Derivation of Genea052 human embryonic stem cell line. Stem Cell Res. 2016;16(2):327-30.
The Genea052 human embryonic stem cell line was derived from a donated, fully commercially consented ART blastocyst, through ICM outgrowth on inactivated human feeders. The line showed pluripotent cell morphology and genomic analysis verified a 46, XY karyotype and male allele pattern through CGH and STR analysis. Pluripotency of Genea052 was demonstrated with 85% of cells expressing Nanog, 87% Oct4, 60% Tra1-60 and 97% SSEA4, a PluriTest Pluripotency score of 27. 21, Novelty score of 1. 2 and tri-lineage teratoma formation. The cell line was negative for Mycoplasma and any visible contamination.