Effect of short-term estrogen therapy on endothelial function: a double-blinded, randomized, controlled trial.

Climacteric : the journal of the International Menopause Society

PubMedID: 27427235

Hurtado R, Celani M, Geber S. Effect of short-term estrogen therapy on endothelial function: a double-blinded, randomized, controlled trial. Climacteric. 2016;1-4.
OBJECTIVE
To evaluate the effect of short-term hormone replacement therapy with 0.625?mg conjugated estrogens daily on endothelial function of healthy postmenopausal women, using flow-mediated dilation (FMD) of the brachial artery.

METHODS
We performed a double-blinded, randomized, controlled trial over 3 years. Randomization was performed using computer-generated sorting. All participants were blinded to the use of conjugated equine estrogens (CEE) or placebo and FMD was assessed by a blinded examiner, before and after 28 days of medication. A total of 64 healthy postmenopausal women were selected and randomly assigned into two groups of treatment: 0.625?mg of CEE or placebo.

RESULTS
FMD values were statistically different between the groups (p?=?0.025): the group receiving CEE showed a FMD value of 0.011 compared to the placebo group (FMD?=?-0.082). The two groups were additionally evaluated for homogeneity through the Shapiro-Wilk test in respect to variables that could interfere with endothelial function such as age (p?=?0.729), body mass index (p?=?0.891), and time since menopause (p?=?0.724). Other variables were excluded during selection of the participants such as chronic vascular conditions, smoking, and sedentary lifestyle.

CONCLUSION
Our results demonstrate that the administration of 0.625?mg CEE for 28 days is effective in improving vascular nitric oxide-dependent dilation assessed by FMD of the brachial artery in postmenopausal women.

CLINICAL TRIAL REGISTRATION
NCT01482416.