Preparation and Biochemical Property of Penicillin G amidase-loaded Alginate and Alginate/Chitosan Hydrogel Beads.

Recent patents on biotechnology

PubMedID: 27494735

Debnath M, Nupur N, Itbhu A. Preparation and Biochemical Property of Penicillin G amidase-loaded Alginate and Alginate/Chitosan Hydrogel Beads. Recent Pat Biotechnol. 2016;.
BACKGROUND
Penicillin G amidase (PGA) (EC 3.5.1.11) are group of enzymes that mainly involved in synthesis of semi-synthetic ß-lactam antibiotics. Soluble PGA is costly and lacks long term operational stability.

OBJECTIVE
Present work aim to do comparative Biochemical Property study of PGA enzyme immobilized in two hydro-gel beads - Ca-alginate and alginate+chitosan hybrid and morphologically characterised by SEM.

METHODS
PGA immobilized in alginate+chitosan shows high pH and thermal stability. Km, Vmax and Effectiveness factor (?) value of free PGA were 56.19 mg/ml, 1.786 U/ml and 1 respectively. These parameters for PGA immobilized alginate bead were 64.84 mg/ml, 0.781U/ml and 0.437 respectively and for PGA immobilized alginate+chitosan hybrid beads were 87.08 mg/ml, 0.622 U/ml and 0.348 respectively.

RESULTS
Immobilized PGA in alginate+chitosan hybrid beads gave the highest thermal stability, reusability and storage stability than alginate immobilized PGA.

CONCLUSION
The entrapment of PGA onto alginate+chitosan hybrid beads revealed several advantages and could be used in 6APA (6-aminopenicillanic acid) production.