Inactivation, lysis and degradation by-products of Saccharomyces cerevisiae by electrooxidation using DSA.

Environmental science and pollution research international

PubMedID: 27495919

Trigueiro LF, Silva LM, Itto LA, Oliveira TM, Motheo AJ, Martínez-Huitle CA, Alves JJ, Castro SS. Inactivation, lysis and degradation by-products of Saccharomyces cerevisiae by electrooxidation using DSA. Environ Sci Pollut Res Int. 2016;.
The yeast Saccharomyces cerevisiae, a microorganism with cell walls resistant to many types of treatments, was chosen as a model to study electrochemical disinfection process using dimensionally stable anodes (DSA). DSA electrodes with nominal composition of Ti/RuO2TiO2 and Ti/RuO2TiO2IrO2 were evaluated in 0. 05 mol L(-1) Na2SO4 containing yeast. THE RESULTS
showed inactivation about of 100 % of the microorganisms at Ti/RuO2TiO2 by applying 20 and 60 mA cm(-2) after 120 min of electrolysis, while a complete inactivation at Ti/RuO2IrO2TiO2 electrode was achieved after 180 min at 60 mA cm(-2).When chloride ions were added in the electrolyte solution, 100 % of the yeast was inactivated at 20 mA cm(-2) after 120 min of electrolysis, independent of the anode used. In the absence of chloride, the energy consumption (EC) was of 34. 80 kWh m(-3), at 20 mA cm(-2) by using Ti/RuO2TiO2 anode. Meanwhile, in the presence of chloride, EC was reduced, requiring 30. 24 and 30. 99 kWh m(-3) at 20 mA cm(-2), for Ti/RuO2TiO2 and Ti/RuO2IrO2TiO2 electrodes, respectively, The best performance for cell lysis was obtained in the presence of chloride with EC of 88. 80 kWh m(-3) (Ti/RuO2TiO2) and 91. 85 kWh m(-3) (Ti/RuO2IrO2TiO2) to remove, respectively, 92 and 95 % of density yeast.

THE RESULTS
clearly showed that yeast, as a model adopted, was efficiently inactivated and lysed by electrolysis disinfection using DSA-type electrodes.