Mycobacterium oryzae sp. nov., a scotochromogenic, rapidly growing species is able to infect human macrophage cell line.

International journal of systematic and evolutionary microbiology

PubMedID: 27499106

E V V R, A R, S B, Ch S, Ch V R. Mycobacterium oryzae sp. nov., a scotochromogenic, rapidly growing species is able to infect human macrophage cell line. Int J Syst Evol Microbiol. 2016;.
Gram-stain-positive, acid-fast positive, rapidly growing, rod shaped bacteria (designated as strains JC290T, JC430 and JC431) were isolated from paddy cultivated soils on the Western Ghats of India. The phylogenetic analysis placed the three strains among the rapidly growing mycobacteria, being most closely related to Mycobacterium tokaiense 47503T (98. 8%), Mycobacterium murale MA112/96T (98. 8%) and a few other Mycobacterium spp. The level of DNA-DNA reassociation of the three strains with M. tokaiense DSM44635T was 23. 4 ± 4% (26. 1 ± 3%, reciprocal analysis) and 21. 4±2 % (22. 1±4 %, reciprocal analysis). All three strains have >99. 9 % sequence similarity and showed DNA reassociation >85%. Further, phylogenetic analysis based on concatenated sequences (3071bp) of four housekeeping genes (16S rRNA, hsp65, rpoB and sodA), revealed that strain JC290T is clearly distinct from every other Mycobacterium spp. All the three strains have diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositolmannoside (PIM1-3), unidentified phospholipids (PL1-3), unidentified glycolipids (GL1-4) and an unidentified lipid as polar lipids. The predominant isoprenoid quinone for all the three strains was MK-9(H2). Fatty acids were C17:17c, C16:0, C18:19c, C16:17c/C16:16c and C19:17c/C19:16c for all the three strains. On the basis of phenotypic, chemotaxonomic characteristics and phylogenetic data, it was concluded that strain JC290T as member of a novel species within the genus Mycobacterium and for which the name Mycobacterium oryzae sp. nov. is proposed. The type strain is JC290T (= KCTC 39560T = LMG 28809T), while JC430 and JC431 are additional strains.