Effects of seed priming, salinity and methyl jasmonate treatment on bioactive composition of Brassica Oleracea var.capitata (white and red varieties) sprouts.

Journal of the science of food and agriculture

PubMedID: 27625158

Hassini I, Baenas N, Moreno DA, Carvajal M, Boughanmi N, Martinez-Ballesta MD. Effects of seed priming, salinity and methyl jasmonate treatment on bioactive composition of Brassica Oleracea var.capitata (white and red varieties) sprouts. J Sci Food Agric. 2016;.
BACKGROUND
Brassica spp. sprouts are rich in nutrients and bioactive compounds, especially glucosinolates and phenolic acid derivatives, and the composition of these young germinating seeds can be altered by several external factors. In this study two cabbage varieties (Brassica oleracea var.capitata, red and white) were studied using seed priming (KCl 50 mM; NaCl 150 mM) and MeJA spraying (25┬ÁM) to elicit the phytochemical content of the edible sprouts.

RESULTS
The red variety was richer in glucosinolates and phenolic compounds than the white one but not in mineral nutrients. Seed priming enhanced the potassium (K) content and the flavonols in both varieties, while the total content of glucosinolates were reduced after seed priming only in the red variety. The white variety responded better than the red one to KCl seed priming, increasing the flavonols (89%). Salinity did not induce any change in the phytochemical contents of these two varieties. The elicitation with sprayed MeJA was effective to significantly increase the content of indolic glucosinolates glucobrassicin (5.7-fold) and neoglucobrassicin (9.7-fold) in the red cultivar. In the white variety, in addition to glucobrassicin (19.4-fold) and neoglucobrassicin (9.4-fold), 4-hydroxyglucobrassicin (2.3-fold) was also enhanced. MeJA elicited also the amounts of anthocyanins (41%) and chlorogenic acid derivatives (329%) in the white variety.

CONCLUSION
KCl seed priming and MeJA elicitation promoted the phytochemical composition of the cabbage varieties, especially in the white variety. The application of NaCl resulted in less efficient elicitation.