Smart Bomb AS1411 aptamer-functionalized/PAMAM dendrimer nanocarriers for targeted drug delivery in the treatment of gastric cancer.

Clinical and experimental pharmacology & physiology

PubMedID: 27626786

Barzegar Behrooz A, Nabavizadeh F, Adiban J, Shafiee Ardestani M, Vahabpour R, Aghasadeghi MR, Sohanaky H. Smart Bomb AS1411 aptamer-functionalized/PAMAM dendrimer nanocarriers for targeted drug delivery in the treatment of gastric cancer. Clin Exp Pharmacol Physiol. 2016;.
Chemotherapy, a conventional method assessed in recent oncology studies, poses numerous problems in the clinical environment. To overcome the problems inherent in chemotherapy, an intelligent drug delivery system has come to the forefront of cancer therapeutics. In this study, we designed a dendrimer-based pharmaceutical system together with a single-stranded AS1411 aptamer (APT(AS) (1411) ) as a therapeutic strategy. The PAMAM (polyamidoamine)-PEG (polyethylene glycol) complex was then conjugated with the AS1411 aptamer and confirmed by AFM (Atomic-force microscopy) and FTIR (Fourier transform infrared spectroscopy). In this study, we show that the conjugated PAMAM-PEG-APT(AS) (1411) complex dramatically increased PAMAM-PEG-5-FU uptake by MKN45 gastric cancer cells. We also demonstrated both the stability of the nanoparticle-5-FU-APT(AS) (1411) complex, by thin layer chromatography (TLC), and an increase in 5-FU (5-fluorouracil) accumulation in the vicinity of cancerous tumors. This smart drug delivery system is capable of effectively transferring 5-FU to MKN45 gastric cancer cells in consistent and without toxic effects. This article is protected by copyright. All rights reserved.