ANNALS EXPRESS: Identification of Hb New York by HbA1c measurement using the Sebia Capillarys 2 Flex Piercing system.

Annals of clinical biochemistry

PubMedID: 27629909

Chao Y, Wan Z, Wu X, Qiu F, Wu X, Wang Y, Ke P, Xu J, Zhuang J, Huang X. ANNALS EXPRESS: Identification of Hb New York by HbA1c measurement using the Sebia Capillarys 2 Flex Piercing system. Ann Clin Biochem. 2016;.
Haemoglobinopathies may interfere with the hemoglobin A1 c (HbA1 c) measurement, leading to incorrect diagnosis and inappropriate treatment. It is essential that HbA1 c assays are capable of identifying haemoglobinopathies. We report two cases of haemoglobin New York (HbNY) discovered through HbA1 c analysis using capillary electrophoresis (Capillarys 2 Flex Piercing [C2FP], Sebia). We used these samples to evaluate the ability of three other HbA1 c assays to identify this variant: ion-exchange high performance liquid chromatography (HPLC; Variant II Turbo [VII-T], Bio-Rad); boronate affinity HPLC (Ultra(2), Trinity Biotech); and immunoassay (cobas c501 Tina-quant Generation 3, Roche Diagnostics). Each method was used for HbA1 c assay of in samples from two cases of heterozygous haemoglobinopathy: ß(0)-thalassemia/ HbNY (Case 1); and HbA/NY (Case 2). Only the C2FP system detected HbNY (an additional peak appeared between HbA1 c and HbA0). CLINICAL
laboratories should be aware of the limitations of their HbA1 c assay methods especially in geographic areas where haemoglobinopathy prevalence is high.