Glaucocalyxin A Ameliorates Myocardial Ischemia-Reperfusion Injury in Mice by Suppression of Microvascular Thrombosis.

Medical science monitor : international medical journal of experimental and clinical research

PubMedID: 27716735

Liu X, Xu D, Wang Y, Chen T, Wang Q, Zhang J, You T, Zhu L. Glaucocalyxin A Ameliorates Myocardial Ischemia-Reperfusion Injury in Mice by Suppression of Microvascular Thrombosis. Med Sci Monit. 2016;223595-3604.
BACKGROUND
The aim of this study was to evaluate the cardio-protective roles of glaucocalyxin A (GLA) in myocardial ischemia-reperfusion injury and to explore the underlying mechanism.

MATERIAL AND METHODS
Myocardial ischemia-reperfusion in wild-type C57BL/6J mice was induced by transient ligation of the left anterior descending artery.GLA or vehicle (solvent) was administrated intraperitoneally to the mice before reperfusion started. After 24 h of myocardial reperfusion, ischemic size was revealed by Evans blue/TTC staining. Cardiac function was evaluated by echocardiography and microvascular thrombosis was assessed by immunofluorescence staining of affected heart tissue. We also measured the phosphorylation of AKT, ERK, P-GSK-3ß, and cleaved caspase 3 in the myocardium.

RESULTS
Compared to the solvent-treated control group, GLA administration significantly reduced infarct size (GLA 13.85±2. 08% vs. Control 18. 95±0. 97%, p<0. 05) and improved left ventricular ejection fraction (LVEF) (GLA 53. 13±1. 11% vs. Control 49. 99±1. 25%, p<0. 05) and left ventricular fractional shortening (LVFS) (28. 34±0. 71% vs. Control 25. 11±0. 74%, p<0. 05) in mice subjected to myocardial ischemia-reperfusion. GLA also attenuated microvascular thrombosis (P<0. 05) and increased the phosphorylation of pro-survival kinase AKT (P<0. 05) and GSK-3ß (P<0. 05) in the myocardium upon reperfusion injury.

CONCLUSIONS
Administration of GLA before reperfusion ameliorates myocardial ischemia-reperfusion injury in mice.The cardio-protective roles of GLA may be mediated through the attenuation of microvascular thrombosis.