Human umbilical cord derived matrix: A scaffold suitable for tissue engineering application.

Bio-medical materials and engineering

PubMedID: 28372283

Dan P, Velot É, Mesure B, Groshenry G, Bacharouche J, Decot V, Menu P. Human umbilical cord derived matrix: A scaffold suitable for tissue engineering application. Biomed Mater Eng. 2017;28(s1):S95-S100.
BACKGROUND
Human tissue derived natural extracellular matrix (ECM) has great potential in tissue engineering.

OBJECTIVE
We sought to isolate extracellular matrix derived from human umbilical cord and test its potential in tissue engineering.

METHODS
An enzymatic method was applied to isolate and solubilized complete human umbilical cord derived matrix (hUCM). The obtained solution was analyzed for growth factors, collagen and residual DNA contents, then used to coat 2D and 3D surfaces for cell culture application.

RESULTS
The hUCM was successfully isolated with trypsin digestion to acquire a solution containing various growth factors and collagen but no residual DNA. This hUCM solution can form a coating on 2D and 3D substrates suitable cell culture.

CONCLUSION
We developed a new matrix derived from human source that can be further used in tissue engineering.