Fowl Adenovirus-Based Vaccine Platform.

Methods in molecular biology (Clifton, N.J.)

PubMedID: 28374242

Corredor JC, Pei Y, Nagy É. Fowl Adenovirus-Based Vaccine Platform. Methods Mol Biol. 2017;158129-54.
Nonpathogenic fowl adenoviruses (FAdVs) are amenable for engineering multivalent vaccine platforms due to large stretches of nonessential DNA sequences in their genomes. We describe the generation of FAdV-9-based vaccine platforms by targeted homologous recombination in an infectious clone (pPacFAdV-9 or wild type FAdmid) containing the entire viral genome in a cosmid vector. The viral DNA is subsequently released from the cosmid by restriction enzyme digestion followed by transfection in a chicken hepatoma cell line (CH-SAH). Virus is harvested, propagated, and verified for foreign gene expression.