Burkholderia thailandensis: Genetic Manipulation.

Current protocols in microbiology

PubMedID: 28510362

Garcia EC. Burkholderia thailandensis: Genetic Manipulation. Curr Protoc Microbiol. 2017;454C.2.1-4C.2.15.
Burkholderia thailandensis is a Gram-negative bacterium endemic to Southeast Asian and northern Australian soils. It is non-pathogenic; therefore, it is commonly used as a model organism for the related human pathogens Burkholderia mallei and Burkholderia pseudomallei. B. thailandensis is relatively easily genetically manipulated and a variety of robust genetic tools can be used in this organism. This unit describes protocols for conjugation, natural transformation, mini-Tn7 insertion, and allelic exchange in B. thailandensis. © 2017 by John Wiley & Sons, Inc.