[Effect of Buyang Huanwu decoction on NF-?B/MuRFl expression in rats with denervated tibial muscle atrophy].

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica

PubMedID: 29082703

Zhou L, Mei XY. [Effect of Buyang Huanwu decoction on NF-?B/MuRFl expression in rats with denervated tibial muscle atrophy]. Zhongguo Zhong Yao Za Zhi. 2017;42(9):1762-1765.
We aim to study the effect of Buyang Huanwu decoction (Astragali Radix, Angelicae Sinensis Radix, Paeoniae Radix Alba, Pheretima, Chuanxiong Rhizoma, Carthami Flos and Persicae Semen) on NF-?B/MuRFl signaling pathway in the denervated tibial muscle atrophy of rats and explore its protection mechanisms. Sixty Sprague-Dawley rats were subjected to common peroneal nerve crush models of 5 mm injury, and then were randomly divided into six groups for daily intragastric administration of drugs: sham operation group, model group, Buyang Huanwu decoction high, middle and low dose groups, and mecobalamin group (as a positive control). After drug administration for 10 days and 21 days, the gene and protein expression levels of NF-?B and MuRFl in the tibial muscle of rats were detected by qRT-PCR and Western blot respectively. THE RESULTS
showed that, as compared with the model group, RNA and protein expression levels of NF-?B and MuRF1 were significantly reduced in Buyang Huanwu decoction middle and high-dose groups (P<0.05 or P<0. 01). These results indicated that Buyang Huanwu decoction could inhibit ubiquitin proteasome system probably by activating NF-?B/MuRF1 signal pathway to protect the denervated tibial muscle atrophy of rats.