Next-generation sequencing for preimplantation genetic diagnosis

Fertility and Sterility

We read with interest the editorial written by Simpson et al. regarding next-generation sequencing (NGS) for preimplantation genetic diagnosis (PGD) . Although the authors provide their debatable opinion regarding the history, success, and failure of earlier and existing methodologies for genetic analysis of the human embryo, here we focus only on the major concern raised over allele dropout (ADO) after using NGS, and the proposed limitations of NGS in PGD, which are based on inaccurate assumptions of the methodology and a misrepresentation of contemporary PGD.