Purification, characterization and antiviral activity of two heteropolysaccharides from Duchesneae Indicae.

International journal of biological macromolecules

PubMedID: 23628582

Jiang H, Wang J, Zhou A, Xie G, Yan L, Jiang Y, Chen J, Shen Y, Chen J. Purification, characterization and antiviral activity of two heteropolysaccharides from Duchesneae Indicae. Int J Biol Macromol. 2013;58287-95.
This study was designed to uncover the antiviral compounds contained in Duchesneae Indicae. Two non-sulfated acidic heteropolysaccharides (DIP30 and DIP60) with anti-varicella-zoster virus (anti-VZV) activity were purified and investigated with a combination of DEAE C-52 ion-exchange chromatography, Sphacryl S-300 gel chromatography, high performance size exclusion chromatography, pre-column derivation with 1-phenyl-3-methyl-5-pyrazolone (PMP) method, infrared (IR) spectroscopy, (1)H and (13)C nuclear magnetic resonance (NMR). Results indicated that molecular weight (MW) of DIP30 and DIP60 are 1.98×10(3) kDa and 34 kDa, respectively. Both DIP30 and DIP60 have a backbone of ß-conformation and consist of more than four kinds of monosaccharides. The anti-VZV 50% effective concentrations (EC50) of DIP30 and DIP60 are 265.2±35.4 µg/ml and 325.6±42 µg/ml, respectively, suggesting that DIP30 and DIP60 could be explored as novel antiviral agents.