A novel acetylcholine bioensor and its electrochemical behavior.

Journal of biomedical nanotechnology

PubMedID: 23621036

Wang W, Deng Y, Li S, Liu H, Lu Z, Zhang L, Lin L, Xu L. A novel acetylcholine bioensor and its electrochemical behavior. J Biomed Nanotechnol. 2013;9(4):736-40.
A novel type of acetylcholine electrochemical biosensor was successfully fabricated by dropping beta-cyclodextrin solution, acetylcholinesterase solution and Nafion-methanol solution to the homemade nano-porous pseudo carbon paste electrode (nano-PPCPE) as working electrode, and then the electrochemical behavior of the as-prepared biosensor was studied through the acetylthiocholine iodide (ATChI). It was shown that the modified nano-PPCPE displays a high selectivity and sensitivity. The acetylcholine biosensor showed the linear range from 5 x 10(-6) M to 2 x 10(-4) M with the detection limit of 1.5 x 10(-7) M.