Synthesis of a novel benzodifuran derivative and its molecular recognition of poly rA RNA.

Biological chemistry

PubMedID: 23729622

Roviello GN, Roviello V, Musumeci D, Pedone C. Synthesis of a novel benzodifuran derivative and its molecular recognition of poly rA RNA. Biol Chem. 2013;394(9):1235-9.
Abstract In this manuscript, we describe a synthetic approach to a novel benzodifuran derivative as well as CD studies regarding its ability to interact with DNA and RNA. After the chemical synthesis and ESI-MS and NMR characterization, this heteroaromatic molecule was investigated by CD spectroscopy in order to evaluate it as a potential nucleic acid binder. Interestingly, the benzodifuran compound was found to be able to induce conformational changes in both DNA and RNA homoadenine molecules forming in the latter case a complex with a 6:1 benzodifuran/nucleobase stoichiometric ratio, as evidenced by CD titration experiments.