Magnetomotive Optical Coherence Tomography for the Assessment of Atherosclerotic Lesions Using avß 3 Integrin-Targeted Microspheres.

Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging

PubMedID: 23907212

Kim J, Ahmad A, Marjanovic M, Chaney EJ, Li J, Rasio J, Hubler Z, Spillman D, Suslick KS, Boppart SA. Magnetomotive Optical Coherence Tomography for the Assessment of Atherosclerotic Lesions Using avß 3 Integrin-Targeted Microspheres. Mol Imaging Biol. 2013;.
PURPOSE
We investigated the early-stage fatty streaks/plaques detection using magnetomotive optical coherence tomography (MM-OCT) in conjunction with avß3 integrin-targeted magnetic microspheres (MSs). The targeting of functionalized MSs was investigated by perfusing ex vivo aortas from an atherosclerotic rabbit model in a custom-designed flow chamber at physiologically relevant pulsatile flow rates and pressures.

PROCEDURES
Aortas were extracted and placed in a flow chamber. Magnetic MS contrast agents were perfused through the aortas and MM-OCT, fluorescence confocal, and bright field microscopy were performed on the ex vivo aorta specimens for localizing the MSs.

RESULTS
The results showed a statistically significant and stronger MM-OCT signal (3.30?±?1.73 dB) from the aorta segment perfused with targeted MSs, compared with the nontargeted MSs (1.18?±?0.94 dB) and control (0.78?±?0.41 dB) aortas. In addition, there was a good co-registration of MM-OCT signals with confocal microscopy.

CONCLUSIONS
Early-stage fatty streaks/plaques have been successfully detected using MM-OCT in conjunction with avß3 integrin-targeted magnetic MSs.