Acceleration of robust "biotube" vascular graft fabrication by in-body tissue architecture technology using a novel eosin Y-releasing mold.

Journal of biomedical materials research. Part B, Applied biomaterials

PubMedID: 23908123

Nakayama Y, Tsujinaka T. Acceleration of robust "biotube" vascular graft fabrication by in-body tissue architecture technology using a novel eosin Y-releasing mold. J Biomed Mater Res Part B Appl Biomater. 2013;.
A novel eosin Y-releasing mold was designed to accelerate the fabrication of in vivo tissue engineered autologous vascular prosthetic tissues, called the "biotubes." The mold was prepared by addition of an aqueous solution of eosin Y (1~6 w/v%) to the agar gel (0.3%), which was attached to the luminal surface of the microporous acrylate tube (diameter, 5 mm; length, 28 mm; pore size, 0.5 mm?). The eosin Y release period was controlled by the number of pores (3~160). On embedding the molds into dorsal, subcutaneous pouches of rats for 1 week, completely encapsulated biotubes, mainly consisting of collagen, with thick walls (418.2?±?173.4?µm) and robust mechanical properties (elastic modulus, 956.2?±?196.5 kPa; burst pressure 5850?±?2383 mmHg) were formed. These values were, respectively, more than 4.3, 3.8, and 5.6 times greater than the corresponding controls (acrylate rods). The high elastic modulus of the biotubes was obtained even with a small number of micropores (3), and a low concentration of eosin Y (1%) within a very short embedding period (5 days), irrespective of rat weights. This innovative method for rapid production of vascular grafts with thick walls and robust mechanical properties may be adaptable for the sub-emergency clinical use of biotubes in regenerative medicine. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2013.