An effective method for determining the ingredients of Shuanghuanglian formula in blood samples using high-resolution LC-MS coupled with background subtraction and a multiple data processing approach.

Journal of separation science

PubMedID: 23913749

Yan GL, Zhang AH, Sun H, Han Y, Shi H, Zhou Y, Wang XJ. An effective method for determining the ingredients of Shuanghuanglian formula in blood samples using high-resolution LC-MS coupled with background subtraction and a multiple data processing approach. J Sep Sci. 2013;.
Shuanghuanglian formula (SF) is a combination of Flos lonicerae japonicae, Radix scutellariae and Fructus forsythiae, commonly used to treat viral or bacterial infections. However, the constituents absorbed into the blood after oral administration of SF are difficult to determine and thus remain unclear. Here we report the application of an accurate background-subtraction and multiple-data-processing approach (Bs-Mpa) for the comprehensive detection of compounds of SF in vivo. A sensitive and reliable ultra-performance liquid chromatography coupled with electrospray-ionization quadrupole time-of-flight mass spectrometry (UPLC-ESI-Q-TOF-MS) approach coupled with Bs-Mpa, which is implemented in the Strip tool from UPLC to remove non-related ion signals from accurate mass LC-MS data, was established to characterize the chemical constituents and rat metabolites of SF. In the loading plot of the principal component analysis, 68 ions of interest were extracted from blood samples, among them, 39 absorbed prototype components of SF and 29 metabolites were identified in vivo. It is concluded that the integrative Bs-Mpa method can be successfully applied for the rapid discovery of multiple components from a traditional Chinese medicine. The above challenge was addressed by using the proposed Bs-Mpa method and it was particularly suitable for applying to the global characterization of the constituents or metabolites in rat blood after oral administration of other well-known formulae. This article is protected by copyright. All rights reserved.