Small-scale extraction of recombinant proteins from bacteria.

Cold Spring Harbor protocols

PubMedID: 20810630

Simpson RJ. Small-scale extraction of recombinant proteins from bacteria. Cold Spring Harb Protoc. 2010;2010(9):pdb.prot5483.
Bacteria are particularly convenient for producing recombinant proteins for purification purposes. To monitor induction as well as the levels of recombinant protein expression, it is important to have a rapid, simple method for estimating bacterial protein expression. This protocol describes the preparation of small-scale bacterial extracts using cell lysis with 0.5% Triton X-100.