Influence of an autologous serum-supplemented medium on the proliferation and differentiation into neurons of canine bone marrow stromal cells.

The Journal of veterinary medical science / the Japanese Society of Veterinary Science

PubMedID: 22293468

Edamura K, Kurosawa T, Nakano R, Teshima K, Asano K, Tanaka S. Influence of an autologous serum-supplemented medium on the proliferation and differentiation into neurons of canine bone marrow stromal cells. J Vet Med Sci. 2012;74(6):817-20.
We investigated the influence of autologous serum (AS)-supplemented medium on the proliferation and differentiation into neurons of canine bone marrow stromal cells (BMSCs). Canine BMSCs were cultured using a-MEM only, a-MEM with 10% fetal bovine serum (FBS), and 5, 10 and 20% AS-supplemented a-MEM. Growth of canine BMSCs was observed in all AS groups. The proliferation capacity of canine BMSCs in the AS groups was similar to that in the FBS group. No significant differences between the FBS and AS groups were observed in the percentage of the cells that changed to the neuron-like morphology and neuron-specific enolase-positive ratio after neuronal differentiation. Canine BMSCs cultured using AS-supplemented medium were able to proliferate and showed neuronal differentiation potency.