Protective effect of saffron extract against doxorubicin cardiotoxicity in isolated rabbit heart.

Pharmaceutical biology

PubMedID: 24003974

Chahine N, Hanna J, Makhlouf H, Duca L, Martiny L, Chahine R. Protective effect of saffron extract against doxorubicin cardiotoxicity in isolated rabbit heart. Pharm Biol. 2013;.
Abstract Context: Anticancer treatments such as anthracyclines are effective; however, they induce cardiotoxicity by releasing radical oxygen species (ROS). Saffron (Crocus sativus; Iridaceae) is a widely used spice with antioxidant properties and numerous health benefits that may provide cardioprotection. Objective: To assess the effect of saffron against acute myocardium damage by anthracyclines compared with electrolysis as a free radical generating system. Materials and methods: According to the Langendorff method, we used the model of an isolated rabbit heart perfused in retrograde. In one set of experiments, ROS was generated by electrolysis of the perfused heart solution (3?mA for 30?min) in the presence and absence of saffron extracts at the optimal dose (10?µg/ml). In another set, we perfused the heart with anthracycline, i.e. 30?µM doxorubicin (Doxo) in the presence and absence of 10?µg/ml saffron extracts. We evaluated cardiodynamics, as well as biochemical and pathological parameters, to emphasize the effectiveness of the treatment with saffron extract using the optimal dose of catalase (150?IU) as a positive control. Results: ROS generated, respectively, by electrolysis and by Doxo significantly (p?