Conservation of fruit dehiscence pathways between Lepidium campestre and Arabidopsis thaliana sheds light on the regulation of INDEHISCENT.

The Plant journal : for cell and molecular biology

PubMedID: 24004048

Lenser T, Theißen G. Conservation of fruit dehiscence pathways between Lepidium campestre and Arabidopsis thaliana sheds light on the regulation of INDEHISCENT. Plant J. 2013;.
The mode of fruit opening is an important agronomical and evolutionary trait that has been studied intensively in the major plant model system Arabidopsis thaliana. Because fruit morphology is highly variable between different species and also often the target of artificial selection during breeding, it is interesting to investigate whether a change in fruit morphology might alter the developmental pathway leading to fruit opening. We study fruit development in Lepidium campestre, a Brassicaceae forming silicles instead of siliques. Transgenic L. campestre plants with altered expression levels of orthologs of A. thaliana fruit developmental genes (ALCATRAZ, FRUITFULL, INDEHISCENT, and SHATTERPROOF1, 2) were found to be defective in fruit dehiscence, and anatomical sections revealed similar changes in tissue patterning as found in respective A. thaliana mutants. Gene expression analyses demonstrated a high degree of conservation in gene regulatory circuits, indicating that despite of the great differences in fruit morphology, the process of fruit opening remains basically unchanged between both species. Interestingly, our data identify ALCATRAZ as a negative regulator of INDEHISCENT in L. campestre. By mutant analysis we found the same regulatory relationship also in A. thaliana, thereby shedding new light on how ALCATRAZ drives separation layer formation. This article is protected by copyright. All rights reserved.