Ultramarine, a chromoprotein acceptor for Förster resonance energy transfer.

PloS one

PubMedID: 22815901

Pettikiriarachchi A, Gong L, Perugini MA, Devenish RJ, Prescott M. Ultramarine, a chromoprotein acceptor for Förster resonance energy transfer. PLoS ONE. 2012;7(7):e41028.
We have engineered a monomeric blue non-fluorescent chromoprotein called Ultramarine (fluorescence quantum yield, 0.001; e(585 nm), 64,000 M(-1) x cm(-1)) for use as a Förster resonance energy transfer acceptor for a number of different donor fluorescent proteins. We show its use for monitoring activation of caspase 3 in live cells using fluorescence lifetime imaging. Ultramarine has the potential to increase the number of cellular parameters that can be imaged simultaneously.