A selective luminescent probe for the direct time-gated detection of adenosine triphosphate.

Journal of the American Chemical Society

PubMedID: 22994413

Weitz EA, Chang JY, Rosenfield AH, Pierre VC. A selective luminescent probe for the direct time-gated detection of adenosine triphosphate. J Am Chem Soc. 2012;134(39):16099-102.
A molecular probe for the luminescent detection of adenosine nucleotides is presented. The probe, Tb-DOTAm-Phen, readily distinguishes among the three adenosine nucleotides in buffered aqueous conditions at neutral pH, a requirement for the direct monitoring of enzymatic reactions converting adenosine triphosphate (ATP) to adenosine diphosphate or adenosine monophosphate. The probe is most efficient under millimolar concentrations of ATP which are relevant to intracellular conditions. Moreover, the long luminescence lifetime of the probe readily enables time-gating experiments.