Characterisation of pks15/1 in clinical isolates of Mycobacterium tuberculosis from Mexico.

Memorias do Instituto Oswaldo Cruz

PubMedID: 24037193

Zenteno-Cuevas R, Silva-Hernández FX, Mendoza-Damián F, Ramírez-Hernández MD, Vázquez-Medina K, Widrobo-García L, Cuellar-Sanchez A, Muñíz-Salazar R, Enciso-Moreno L, Pérez-Navarro LM, Enciso-Moreno JA. Characterisation of pks15/1 in clinical isolates of Mycobacterium tuberculosis from Mexico. Mem Inst Oswaldo Cruz. 2013;108(6):718-23.
Tuberculosis (TB) is an infectocontagious respiratory disease caused by members of the Mycobacterium tuberculosis complex. A 7 base pair (bp) deletion in the locus polyketide synthase (pks)15/1 is described as polymorphic among members of the M. tuberculosis complex, enabling the identification of Euro-American, Indo-Oceanic and Asian lineages. The aim of this study was to characterise this locus in TB isolates from Mexico. One hundred twenty clinical isolates were recovered from the states of Veracruz and Estado de Mexico. We determined the nucleotide sequence of a ± 400 bp fragment of the locus pks15/1, while genotypic characterisation was performed by spoligotyping. One hundred and fifty isolates contained the 7 bp deletion, while five had the wild type locus. Lineages X (22%), LAM (18%) and T (17%) were the most frequent; only three (2%) of the isolates were identified as Beijing and two (1%) EAI-Manila. The wild type pks15/1 locus was observed in all Asian lineage isolates tested. Our results confirm the utility of locus pks15/1 as a molecular marker for identifying Asian lineages of the M. tuberculosis complex. This marker could be of great value in the epidemiological surveillance of TB, especially in countries like Mexico, where the prevalence of such lineages is unknown.