[Protective effect of tert-butylhydroquinone on PC12 cells from neurotoxicity induced by manganese in vitro].

Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases

PubMedID: 20137389

Li HY, Wu SY, Lin W, Zhou WH, Zhang WC, Li T, Shi N. [Protective effect of tert-butylhydroquinone on PC12 cells from neurotoxicity induced by manganese in vitro]. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2009;27(10):597-600.
OBJECTIVE
To investigate the protective effect of the tert-butylhydroquinone (tBHQ) on PC12 cells from neurotoxicity induced by manganese.

METHODS
Cytotoxicity of PC12 cells was measured by MTT assay, following the PC12 cells treatment with different concentrations of MnCl2 (300, 600, 900 µmol/L) for 24, 48 or 72 h. PC12 cells were pretreated with 40 µmol/L tBHQ for 12 h, followed by the treatment of 600 micromol/L or 300 µmol/L MnCl2 for 72 h. Cytotoxicity of PC12 cells was measured by MTT assay, and cell apoptosis was examined by the method of Annexin V-FITC/PI in flow cytometry (FCM).

RESULTS
The proliferation of PC12 cells treated with 300, 600, 900 µmol/L MnCl2 was suppressed in the dose dependent pattern (P < 0.01). Proliferation of PC12 cells treated with 600 µmol/L MnCl2 was suppressed to 40% of that in control group (P < 0.01), but the proliferation rate of PC12 cell pretreated with 40 µmol/L tBHQ was 180% of that in control group (P < 0.01). Apoptotic rate of PC12 cells treated with 300 micromol/L MnCl2 was higher than the vehicle control group (P < 0.01). Apoptotic rate of 40 µmol/L tBHQ pretreatment followed by 300 µmol/L MnCl2 treatment was lower than that of MnCl2 treatment group (P < 0.01). The inhibition rate of apoptosis was 61%.

CONCLUSIONS
Manganese may suppress PC12 cells proliferation and induce apoptosis. tBHQ can reduce PC12 cells proliferation suppressed by manganese and attenuate the apoptosis induced by manganese.