Magnetic-fluorescent colloidal nanobeads: preparation and exploitation in cell separation experiments.

Macromolecular bioscience

PubMedID: 19593784

Di Corato R, Piacenza P, MusarĂ² M, Buonsanti R, Cozzoli PD, Zambianchi M, Barbarella G, Cingolani R, Manna L, Pellegrino T. Magnetic-fluorescent colloidal nanobeads: preparation and exploitation in cell separation experiments. Macromol Biosci. 2009;9(10):952-8.
Nanostructures displaying fluorescence and magnetic properties at the same time are potentially useful for achieving simultaneous bio-separation and bio-sensing (e.g., magnetic separation coupled with multiplexing optical detection of different tumour cell populations). Spherical nanobeads that display both fluorescent and magnetic features are reported; they are fabricated by grafting fluorescent oligothiophene molecules to an amphiphilic polymer that is then used to enwrap iron oxide nanoparticles, which acts as the magnetic domain. By tuning experimental conditions, control over the number of magnetic nanoparticles per bead and over the bead diameter (30-400 nm) was achieved. A cell separation efficiency of the level required for cell sorting applications is also reported.