Post-flask management of yam (Dioscorea) plantlets.

Cold Spring Harbor protocols

PubMedID: 20150065

Mignouna HD, Abang MM, Asiedu R, Geeta R. Post-flask management of yam (Dioscorea) plantlets. Cold Spring Harb Protoc. 2009;2009(11):pdb.prot5326.
This protocol describes how to harden or acclimatize tissue culture-derived yam (Dioscorea) plantlets before transplanting them in the soil. Briefly, the plantlets are first put in pots with a vermiculite and peat moss mixture, and the pots are placed in a humidity chamber, where they are sprayed and watered daily. After 2 wk, the plants are transplanted to pots or plastic bags with ordinary or sterile soil and watered daily. After another 2 wk, they are transplanted into the field.