Whole Salivary Interleukin-6 and Matrix Metalloproteinase-8 Levels in Chronic Periodontitis Patients With and Without Prediabetes.

Journal of periodontology

PubMedID: 24171502

Javed F, Ahmed HB, Saeed A, Mehmood A, Bain C. Whole Salivary Interleukin-6 and Matrix Metalloproteinase-8 Levels in Chronic Periodontitis Patients With and Without Prediabetes. J Periodontol. 2013;.
Background: Cytokine profile in unstimulated whole saliva (UWS) of prediabetic patients with chronic periodontitis (CP) remains uninvestigated. The aim was to assess interleukin-6 (IL-6) and matrix metalloproteinase-8 (MMP-8) levels in UWS of CP patients with and without prediabetes. Methods: Eighty-eight individuals (age range 39-51 years) were divided into three groups. Group-1: 28 CP patients with prediabetes; Group-2: 30 CP patients without prediabetes; and Group-3: 30 controls. Fasting blood glucose levels (FBGL) and hemoglobin A1c levels (HbA1c), periodontal parameters (plaque index [PI], bleeding on probing [BOP], probing depth [PD], clinical attachment loss [AL] and marginal bone loss) and number of missing teeth (MT) were recorded. UWS samples were collected and unstimulated whole salivary flow rate (UWSFR) was measured. IL-6 and MMP-8 were measured in UWS using enzyme linked immunosorbent assay. P-values less than 0.05 were considered statistically significant. Results: Mean FBGL, HbA1c levels were significantly higher among participants in Group-1 (119.3±3.1mg/dL and 6.1±0.2%) than Group-2 (80.1±3.5mg/dL and 4.8±0.5%) (P<0.001) and Group-3 (75.3±2.2mg/dL and 4.3±0.2%) (P<0.05). UWSFR was significantly higher among individuals in groups 2 (0.53±0.1ml/min) (P<0.05) and 3 (0.51±0.1ml/min) (P<0.01) than patients in Group-1 (0.33±0.05ml/min). Periodontal parameters were worse among patients in Group-1 (P<0.05) and Group-2 (P<0.05) than individuals in Group-3. There was no difference in periodontal parameters, numbers of MT and salivary IL-6 and MMP-8 levels among patients in groups 1 and 2. Conclusion: Salivary IL-6 and MMP-8 levels are elevated in CP patients with and without prediabetes.