Cationically rendered biopolymer surfaces for high protein affinity support matrices.

Chemical communications (Cambridge, England)

PubMedID: 24178232

Mohan T, Ristic T, Kargl R, Doliska A, Köstler S, Ribitsch V, Marn J, Spirk S, Stana-Kleinschek K. Cationically rendered biopolymer surfaces for high protein affinity support matrices. Chem Commun (Camb). 2013;.
The use of cationic biopolymer surfaces for high protein binding affinity matrices is described. As model proteins, fluorescently labeled bovine serum albumins (FITC-BSA, TRITC-BSA) have been employed. The amount of proteins on such cationically rendered surfaces was quantified by QCM-D. In addition, flexible, transparent, patterned COP slides have been prepared and loaded with proteins ranging from 15 pM to 15 µM TRITC-BSA.