Increasing the Scale of Peroxidase Production by Streptomyces sp. strain BSII#1.

Journal of applied microbiology

PubMedID: 24176016

Musengi A, Khan N, Le Roes-Hill M, Pletschke BI, Burton SG. Increasing the Scale of Peroxidase Production by Streptomyces sp. strain BSII#1. J Appl Microbiol. 2013;.
AIMS
To optimise peroxidase production by Streptomyces sp. strain BSII#1, up to 3 l culture volumes.

METHODS AND RESULTS
Peroxidase production by Streptomyces sp. strain BSII#1 was optimised in terms of production temperature and pH and the use of lignin-based model chemical inducers. The highest peroxidase activity (1.30±0.04 U ml(-1) ) in 10 ml culture volume was achieved in a complex production medium (pH 8.0) at 37°C in the presence of 0.1 mmol l(-1) veratryl alcohol, which was greater than those reported previously. Scale-up to 100 ml and 400 ml culture volumes resulted in decreased peroxidase production (0.53±0.10 U ml(-1) and 0.26±0.08 U ml(-1) respectively). However, increased aeration improved peroxidase production with the highest production achieved using an airlift bioreactor (4.76±0.46 U ml(-1) in 3 l culture volume).

CONCLUSIONS
Veratryl alcohol (0.1 mmol l(-1) ) is an effective inducer of peroxidase production by Streptomyces sp. strain BSII#1. However, improved aeration increased peroxidase production in larger volumes without the use of an inducer, surpassing induced yields in an optimised small-scale process.

SIGNIFICANCE AND IMPACT OF THE STUDY
Only a limited number of reports in literature have focused on the up-scaling of bacterial peroxidase production. There remains opportunity for feasible large-scale production of bacterial peroxidases with potentially novel biocatalytic properties. This article is protected by copyright. All rights reserved.