[PDX-1 gene transfection and expression in bone marrow stromal cells-derived nestin-positive cells].

Nan fang yi ke da xue xue bao = Journal of Southern Medical University

PubMedID: 17545051

Wang HL, Pan MX, Zhang HY, An J, Gao Y. [PDX-1 gene transfection and expression in bone marrow stromal cells-derived nestin-positive cells]. Nan Fang Yi Ke Da Xue Xue Bao. 2007;27(4):528-31.
OBJECTIVE
To introduce the eukaryotic expression vector pEGFP-C1-PDX-1 into nestin-positive cell derived from bone marrow stromal cells by nucleofection and optimize the conditions for transfection.

METHODS
The recombinant plasmid was transfected into bone marrow stromal cells-derived nestin-positive cells with varied DNA quantities or the serum concentration in the medium. The expression of PDX-1 gene in the transfected cells was detected by RT-PCR.

RESULTS
Satisfactory efficiency of transfection was achieved with the DNA quantity of 2-10 microg and medium serum concentration of 20%. PDX-1 expression was detected in the transfected cells by RT-PCR.

CONCLUSION
The optimized transfection conditions result in enhanced efficiency of PDX-1 gene transfection into nestin-positive cells derived from bone marrow stromal cells, which may serve as the seed cells in tissue-engineering.