[Complementarity-determining region 3 analysis of T cell receptor beta chain variable region in peripheral blood mononuclear cells cultured with interleukin-2].

Nan fang yi ke da xue xue bao = Journal of Southern Medical University

PubMedID: 17545022

Chang H, Luo W, Ma L, Zhou MQ, Wen Q, Wu YB, Huang YX, Guo KY. [Complementarity-determining region 3 analysis of T cell receptor beta chain variable region in peripheral blood mononuclear cells cultured with interleukin-2]. Nan Fang Yi Ke Da Xue Xue Bao. 2007;27(4):433-5.
OBJECTIVE
To analyze the drift of the complementarity-determining region 3 (CDR3) of T cell receptor beta (TCRbeta) chain variable region in T cells of healthy volunteers cultured with interleukin-2 (IL-2).

METHODS
T cells were isolated from the peripheral blood and cultured in vitro in the presence of IL-2. The non-specific killing effect of the cells was analyzed by LDH releasing assay, and the distribution of TCRbeta chain CDR3 in healthy volunteers by immunoscope spectratyping method to evaluate the clonality of the T cells.

RESULTS
The results showed Gaussian distribution of TCR Vbeta gene CDR3 in healthy volunteers. The T cell cultured with IL-2, however, displayed some anomalous and oligoclonal expansion in different TCR Vbeta families without killing effect against nasophargngal carcinoma cell line CNE2.

CONCLUSION
IL-2 may affect TCRbeta chain CDR3 distribution in T cells cultured in vitro.