A rapid and simple method for DNA extraction from yeasts and fungi isolated from Agave fourcroydes.

Molecular biotechnology

PubMedID: 16691008

Tapia-Tussell R, Lappe P, Ulloa M, Quijano-Ramayo A, Cáceres-Farfán M, Larqué-Saavedra A, Perez-Brito D. A rapid and simple method for DNA extraction from yeasts and fungi isolated from Agave fourcroydes. Mol Biotechnol. 2006;33(1):67-70.
A simple and easy protocol for extracting high-quality DNA from different yeast and filamentous fungal species is described. This method involves two important steps: first, the disruption of cell walls by mechanical means and freezing; and second, the extraction, isolation, and precipitation of genomic DNA. The absorbance ratios (A(260)/A(280)) obtained ranged from 1.6 to 2.0. The main objective of this procedure is to extract pure DNA from yeast and filamentous fungi, including those with high contents of proteins, polysaccharides, and other complex compounds in their cell walls. The yield and quality of the DNAs obtained were suitable for micro/minisatellite primer-polymerase chain reaction (MSP-PCR) fingerprinting as well as for the sequence of the D1/D2 domain of the 26S rDNA.