Pro-antibiotic substrates for the identification of enantioselective hydrolases.

Biotechnology letters

PubMedID: 16816894

Hwang BY, Oh JM, Kim J, Kim BG. Pro-antibiotic substrates for the identification of enantioselective hydrolases. Biotechnol Lett. 2006;28(15):1181-5.
A new growth-based screening method for the identification of enantioselective hydrolases, such as lipases and esterases, using pro-antibiotic substrates was devised. An enantioselective hydrolase could be identified by measuring growth rates of cells in liquid media containing (R)- or (S)-2-phenylbutyric chloramphenicol esters. This method can be applied to the screening of novel enantioselective microbes and to the high-throughput screening for the directed evolution of enantioselective hydrolytic enzymes.