Synthesis of homogenous site-selectively glycosylated proteins.

Organic & biomolecular chemistry

PubMedID: 15703788

Swanwick RS, Daines AM, Flitsch SL, Allemann RK. Synthesis of homogenous site-selectively glycosylated proteins. Org Biomol Chem. 2005;3(4):572-4.
Apparently homogenous glycoproteins can be synthesised in good yield by a combination of site directed mutagenesis, a highly flexible but selective chemical derivatisation and efficient purification through the use of glycosyl thiosulfonates such as 2-((biotinoyl)-amino)-ethyl methanethiosulfonate.