Standard operating procedure for immunuslotblot assay for analysis of DNA/sulfur mustard adducts in human blood and skin.

Journal of analytical toxicology

PubMedID: 15239849

van der Schans GP, Mars-Groenendijk R, de Jong LP, Benschop HP, Noort D. Standard operating procedure for immunuslotblot assay for analysis of DNA/sulfur mustard adducts in human blood and skin. J Anal Toxicol. 2004;28(5):316-9.
A standard operating procedure has been developed for an immunoslotblot assay of sulfur mustard adducts to DNA in human blood and skin for use in a field laboratory. A minimum detectable level of exposure of human blood in vitro (> or = 50 nM) sulfur mustard is feasible with the assay. In the case of human skin, a 1 s exposure to saturated sulfur mustard vapor (830 mg/m(-3)) could still be detected.