Design and synthesis of a solid-supported FR225659 derivative for its receptor screening.

Organic letters

PubMedID: 15228308

Zenkoh T, Hatori H, Tanaka H, Hasegawa M, Hatakeyama M, Kabe Y, Setoi H, Kawaguchi H, Handa H, Takahashi T. Design and synthesis of a solid-supported FR225659 derivative for its receptor screening. Org Lett. 2004;6(14):2477-80.
[structure: see text] We describe the design and synthesis of latex particles attached to an FR225659 derivative to identify its receptor proteins. Two key building blocks were prepared by two-step degradation of FR225659 under basic conditions. The designed ligand showed an acceptable level of biological activity to make it of potential value for use in affinity-supported receptor identification. Affinity purification of FR225659-binding proteins using the latex nanoparticles provided three candidate receptor peptides for the biological activity.